{"type":"research","record":{"id":2414,"source_id":"europe-pmc","external_id":"42717514","title":"Co-Delivery of Sustained Release Chondroitinase ABC-37 With Human iPSC-Derived Neural Progenitors Promotes Transplant Survival and Functional Recovery in a Rodent Model of Stroke.","authors":"Letko Khait N, Li DX, Guo K, Cui H, Sirianni Q, Abdo D, Morshead CM, Shoichet MS.","journal":null,"published_on":"2026-09-09","doi":"10.1002/advs.202522499","publication_type":null,"evidence_level":"research-record","source_url":"https://europepmc.org/article/MED/42717514","is_open_access":true,"cited_by_count":0,"editorial_summary":"Research record, indexed because it matched monitored longevity research terms. Inclusion in this index does not establish that an intervention is effective or safe.","source_updated_at":"2026-09-16T06:17:02.258+00:00","first_seen_at":"2026-09-15T00:17:12.717774+00:00","last_seen_at":"2026-09-16T06:17:02.258+00:00","status":"published","metadata":{"pmid":"42717514","pmcid":"PMC13558902","source":"MED","has_references":true},"integrity_checked_at":"2026-09-16T06:17:16.341+00:00","abstract_text":"Stroke is a leading cause of disability worldwide, yet effective treatments to regenerate damaged tissue remain elusive. We investigated tissue regeneration and functional recovery post-stroke in an animal model with a combinatorial approach leveraging human induced pluripotent stem cell-derived neural progenitor cells (NPCs) and a sustained-release formulation of a re-designed, thermostable chondroitinase ABC (ChASE37). We optimized an in situ gelling, injectable hyaluronic acid (HA)-based hydrogel with the incorporation of laminin to deliver NPCs to the cavity in a rat model of sub-acute ischemic stroke. We delivered ChASE37 by affinity release from a methylcellulose hydrogel (ChASE37-AR) to the stroke-injured brain by epicortical injection. ChASE37-AR retained long-term enzymatic activity, as demonstrated by degradation of inhibitory chondroitin sulfate proteoglycans (CSPGs) in the injured tissue, with no evidence of immunogenicity. Each treatment - ChASE37-AR, NPCs, and their co-delivery - significantly improved motor function as early as 3 weeks after a single administration. Critically, only the combined therapy supported long-term survival and neuronal differentiation of transplanted cells, suggesting that ChASE37-AR has a role beyond CSPG degradation. This strategy presents a modular, clinically relevant platform for tissue regeneration post-stroke. Together, these findings suggest that the co-delivery of NPCs and sustained-release ChASE37 may be promising for stroke treatment.","controlled_terms":["Hyaluronic acid","Stroke","Chondroitinase ABC","neural progenitor cells","hydrogel"],"relevance_confidence":97,"source_quality_score":90,"freshness_score":100,"publication_state":"published","match_explanation":"Abstract contains controlled term: stem cell, progenitor cell.","quality_checked_at":"2026-09-16T06:17:02.982171+00:00","duplicate_cluster_key":"id:10 1002 advs 202522499","duplicate_of_id":null,"research_item_topics":[{"topic_slug":"stem-cells","is_published":true,"match_reasons":["Abstract contains controlled term: stem cell, progenitor cell.","Source terminology contains: progenitor cell.","Abstract supplies longevity context: aging."],"matched_fields":["abstract","controlled terminology","abstract context"],"relevance_score":97,"intelligence_topics":{"name":"Stem cells","slug":"stem-cells"}}],"content_sources":{"name":"Europe PMC","homepage_url":"https://europepmc.org/"}},"canonical_url":"https://www.immortal.life/research/2414","automation_disclosure":"Generated automatically from cited source metadata. No scientist, clinician, researcher, editor, or human reviewer evaluates this publication before release."}