{"type":"trials","record":{"id":21843,"source_id":"clinicaltrials-gov","external_id":"NCT02132091","title":"Practicality of Intermittent Fasting and Its Effect on Markers of Aging and Oxidative Stress","brief_summary":"Healthy volunteers will be recruited to participate in a ten-week double-blinded crossover trial. The trial will consist of two, three-week periods of intermittent fasting, where subjects receive either antioxidant supplementation or placebo, the ordering of which will be randomly determined. A one-week preconditioning will precede each invention period, and a two week \"wash-out\" period will follow the first intervention period. Serum-based assays will be performed to assess levels of reactive oxidant species, antioxidant genes, sirtuins, and markers of mitochondrial biogenesis and aging. The investigators hypothesize that an intermittent fasting diet in healthy young volunteers will improve these markers of cellular aging and that these beneficial effects will be abrogated by the supplementation of antioxidants. This study is a proof-of-principle study that will shed light on the mechanism and effects of IF as an anti-aging dietary intervention in the absence of weight loss. It will inform the design of dietary interventions that are both effective in improving markers of aging and feasible for patients to practice on a long-term basis.","overall_status":"Completed","phases":[],"study_type":"INTERVENTIONAL","sponsor":"University of Florida","enrollment":37,"countries":["United States"],"start_date":"2011-03-01","completion_date":"2011-11-01","last_update_date":"2015-12-14","source_url":"https://clinicaltrials.gov/study/NCT02132091","editorial_summary":"A registered study indexed because it matched monitored longevity research terms. Registry status: Completed. Registration does not establish safety or effectiveness.","first_seen_at":"2026-09-24T01:17:10.897288+00:00","last_seen_at":"2026-09-25T06:15:54.38+00:00","metadata":{"sex":"ALL","acronym":null,"age_range":"19 Years to 30 Years","comparator":null,"organization":"University of Florida","interventions":["Intermittent Fasting","400 IU Vitamin E","1000 mg Vitamin C"],"registry_source":"ClinicalTrials.gov","outcome_measures":["SOD2 gene expression (intermittent fasting) — 3 weeks","SOD2 gene expression (antioxidant supplementation) — 3 weeks","GPx1 gene expression (intermittent fasting) — 3 weeks","SIRT1 gene expression (intermittent fasting) — 3 weeks","SIRT3 gene expression (intermittent fasting) — 3 weeks","mTFA gene expression (intermittent fasting) — 3 weeks","NRF1 gene expression (intermittent fasting) — 3 weeks","8oxodG ratio (intermittent fasting) — 3 weeks","8oxoG ratio (intermittent fasting) — 3 weeks","GPx1 gene expression (antioxidant supplementation) — 3 weeks","SIRT1 gene expression (antioxidant supplementation) — 3 weeks","SIRT3 gene expression (antioxidant supplementation) — 3 weeks","mFTA gene expression (antioxidant supplementation) — 3 weeks","NRF1 gene expression (antioxidant supplementation) — 3 weeks","8oxodG ratio (antioxidant supplementation) — 3 weeks","8oxoG ratio (antioxidant supplementation) — 3 weeks"],"design_description":"RANDOMIZED · CROSSOVER · BASIC SCIENCE · QUADRUPLE","source_has_results":false},"controlled_terms":["Aging","Metabolism","Intermittent Fasting","400 IU Vitamin E","1000 mg Vitamin C"],"relevance_confidence":100,"source_quality_score":100,"freshness_score":35,"publication_state":"published","match_explanation":"Title contains controlled term: intermittent fasting.","quality_checked_at":"2026-09-25T06:16:07.48358+00:00","duplicate_cluster_key":"id:nct02132091","duplicate_of_id":null,"evidence_snapshot":{"status":"structured","version":1,"duration":"2011-03-01 to 2011-11-01","comparator":null,"confidence":"structured-source","population":"ALL · 19 Years to 30 Years","provenance":{"duration":"start_date and completion_date","comparator":"registry arm fields","population":"registry eligibility fields","intervention":"registry intervention fields","participants":"enrollment","study_design":"study_type and registry design fields","evidence_stage":"phases","reported_outcome":"not available","outcomes_measured":"registry outcome-measure fields"},"generated_at":"2026-09-25T06:15:54.498Z","intervention":["Intermittent Fasting","400 IU Vitamin E","1000 mg Vitamin C"],"participants":37,"study_design":"RANDOMIZED · CROSSOVER · BASIC SCIENCE · QUADRUPLE","subject_scope":"Human clinical study registration","evidence_stage":"Phase not reported","safety_context":"Eligibility, adverse-event details, and clinical decisions must be checked in the official registry and with qualified clinicians.","source_support":"Structured registry protocol metadata; no finding-level conclusion is generated.","main_limitation":"This is a study registration. No reusable structured result is available here, so it cannot show whether the intervention worked or was safe.","reported_outcome":null,"outcomes_measured":["SOD2 gene expression (intermittent fasting) — 3 weeks","SOD2 gene expression (antioxidant supplementation) — 3 weeks","GPx1 gene expression (intermittent fasting) — 3 weeks","SIRT1 gene expression (intermittent fasting) — 3 weeks","SIRT3 gene expression (intermittent fasting) — 3 weeks","mTFA gene expression (intermittent fasting) — 3 weeks","NRF1 gene expression (intermittent fasting) — 3 weeks","8oxodG ratio (intermittent fasting) — 3 weeks","8oxoG ratio (intermittent fasting) — 3 weeks","GPx1 gene expression (antioxidant supplementation) — 3 weeks","SIRT1 gene expression (antioxidant supplementation) — 3 weeks","SIRT3 gene expression (antioxidant supplementation) — 3 weeks","mFTA gene expression (antioxidant supplementation) — 3 weeks","NRF1 gene expression (antioxidant supplementation) — 3 weeks","8oxodG ratio (antioxidant supplementation) — 3 weeks","8oxoG ratio (antioxidant supplementation) — 3 weeks"],"regulatory_context":"Trial registration is not regulatory approval and does not establish that an intervention is available."},"clinical_trial_topics":[{"topic_slug":"caloric-restriction","is_published":true,"match_reasons":["Title contains controlled term: intermittent fasting.","Abstract contains controlled term: intermittent fasting.","Source terminology contains: intermittent fasting.","Title supplies longevity context: aging.","Study type is explicitly identified as INTERVENTIONAL."],"matched_fields":["title","abstract","controlled terminology","title context","study type"],"relevance_score":100,"intelligence_topics":{"name":"Caloric restriction","slug":"caloric-restriction"}},{"topic_slug":"oxidative-stress","is_published":true,"match_reasons":["Title contains controlled term: oxidative stress.","Title supplies longevity context: aging.","Study type is explicitly identified as INTERVENTIONAL."],"matched_fields":["title","title context","study type"],"relevance_score":80,"intelligence_topics":{"name":"Oxidative stress","slug":"oxidative-stress"}}],"content_sources":{"name":"ClinicalTrials.gov","homepage_url":"https://clinicaltrials.gov/"}},"canonical_url":"https://www.immortal.life/trials/21843","automation_disclosure":"Generated automatically from cited source metadata. No scientist, clinician, researcher, editor, or human reviewer evaluates this publication before release."}