{"type":"trials","record":{"id":29458,"source_id":"clinicaltrials-gov","external_id":"NCT02472340","title":"Mitophagy and Autophagy in Elderly Subjects","brief_summary":"In recent years, evidence has shown that mitochondrial dysfunction plays an important role in the development of age-related muscle decline that may lead to frailty. During aging, there is a progressive reduction in the cell's capacity to eliminate its dysfunctional elements by autophagy, as evidenced by the accumulation of oxidative damage and mutations in mitochondria and by the decrease in autophagic flux. In fact, it has been demonstrated that dysfunctional mitochondria can be specifically targeted for elimination by autophagy, a process that has been termed mitophagy. A major challenge in the clinic today is in the lack of validated tools, including biomarkers, to assess the decline in mitochondrial health associated with an impairment in muscle function. In the present study, the investigators will employ a battery of established and exploratory tests (clinical, physiological and molecular) to assess in vivo mitochondrial function and more specifically, the levels of mitophagy and autophagy, in the muscle of healthy and pre-frail elderly. It is anticipated that the results of this study will facilitate the rapid translation of interventions targeting mitophagy and autophagy for the improvement of muscle function.","overall_status":"Completed","phases":[],"study_type":"OBSERVATIONAL","sponsor":"Amazentis SA","enrollment":22,"countries":["Netherlands"],"start_date":"2015-06-01","completion_date":"2016-07-01","last_update_date":"2016-08-01","source_url":"https://clinicaltrials.gov/study/NCT02472340","editorial_summary":"A registered study indexed because it matched monitored longevity research terms. Registry status: Completed. Registration does not establish safety or effectiveness.","first_seen_at":"2026-09-25T01:16:08.862442+00:00","last_seen_at":"2026-09-25T06:05:34.312+00:00","metadata":{"sex":"ALL","acronym":null,"age_range":"61 Years to 85 Years","comparator":null,"organization":"Amazentis SA","interventions":["Muscle Biopsy"],"registry_source":"ClinicalTrials.gov","outcome_measures":["Gene and protein expression for autophagy and mitophagy biomarkers in muscle tissue — 9 months","PCr recovery time (in seconds) measured by 31P-MRS (31-Phosphorus Magnetic Resonance Spectroscopy). — 9 months","mVO2 (in ml/min/100 ml) in muscle measured by NIRS (Near-infrared Spectroscopy) — 9 months","MitoPO2 (in mmHg) in the skin measured by PpIX-TSLT (Protoporphyrin IX - Triplet State Lifetime Technique). — 9 months","Hand grip strength (in kg) measured by the Jamar dynamometer. — 9 months","Peak muscle force of quadriceps measured by handheld dynamometry — 9 months","Postural stability (in mm sway) measured by body sway — 9 months","Level of activity, using the Vital Connect HealthPatch accelerometer — 9 months","Short physical performance battery (SPPB) test. — 9 months"],"design_description":null,"source_has_results":false},"controlled_terms":["Aging","Frailty","Mitophagy","Muscle Biopsy"],"relevance_confidence":100,"source_quality_score":100,"freshness_score":35,"publication_state":"published","match_explanation":"Title contains controlled term: autophagy.","quality_checked_at":"2026-09-25T06:05:35.756693+00:00","duplicate_cluster_key":"id:nct02472340","duplicate_of_id":null,"evidence_snapshot":{"status":"structured","version":1,"duration":"2015-06-01 to 2016-07-01","comparator":null,"confidence":"structured-source","population":"ALL · 61 Years to 85 Years","provenance":{"duration":"start_date and completion_date","comparator":"registry arm fields","population":"registry eligibility fields","intervention":"registry intervention fields","participants":"enrollment","study_design":"study_type and registry design fields","evidence_stage":"phases","reported_outcome":"not available","outcomes_measured":"registry outcome-measure fields"},"generated_at":"2026-09-25T06:05:34.329Z","intervention":["Muscle Biopsy"],"participants":22,"study_design":"OBSERVATIONAL","subject_scope":"Human clinical study registration","evidence_stage":"Phase not reported","safety_context":"Eligibility, adverse-event details, and clinical decisions must be checked in the official registry and with qualified clinicians.","source_support":"Structured registry protocol metadata; no finding-level conclusion is generated.","main_limitation":"This is a study registration. No reusable structured result is available here, so it cannot show whether the intervention worked or was safe.","reported_outcome":null,"outcomes_measured":["Gene and protein expression for autophagy and mitophagy biomarkers in muscle tissue — 9 months","PCr recovery time (in seconds) measured by 31P-MRS (31-Phosphorus Magnetic Resonance Spectroscopy). — 9 months","mVO2 (in ml/min/100 ml) in muscle measured by NIRS (Near-infrared Spectroscopy) — 9 months","MitoPO2 (in mmHg) in the skin measured by PpIX-TSLT (Protoporphyrin IX - Triplet State Lifetime Technique). — 9 months","Hand grip strength (in kg) measured by the Jamar dynamometer. — 9 months","Peak muscle force of quadriceps measured by handheld dynamometry — 9 months","Postural stability (in mm sway) measured by body sway — 9 months","Level of activity, using the Vital Connect HealthPatch accelerometer — 9 months","Short physical performance battery (SPPB) test. — 9 months"],"regulatory_context":"Trial registration is not regulatory approval and does not establish that an intervention is available."},"clinical_trial_topics":[{"topic_slug":"autophagy","is_published":true,"match_reasons":["Title contains controlled term: autophagy.","Abstract contains controlled term: autophagy.","Title supplies longevity context: elderly.","Study type is explicitly identified as OBSERVATIONAL."],"matched_fields":["title","abstract","title context","study type"],"relevance_score":100,"intelligence_topics":{"name":"Autophagy","slug":"autophagy"}},{"topic_slug":"mitochondrial-function","is_published":true,"match_reasons":["Title contains controlled term: mitophagy.","Abstract contains controlled term: mitochondrial dysfunction, mitochondrial function.","Source terminology contains: mitophagy.","Title supplies longevity context: elderly.","Study type is explicitly identified as OBSERVATIONAL."],"matched_fields":["title","abstract","controlled terminology","title context","study type"],"relevance_score":100,"intelligence_topics":{"name":"Mitochondrial function","slug":"mitochondrial-function"}}],"content_sources":{"name":"ClinicalTrials.gov","homepage_url":"https://clinicaltrials.gov/"}},"canonical_url":"https://www.immortal.life/trials/29458","automation_disclosure":"Generated automatically from cited source metadata. No scientist, clinician, researcher, editor, or human reviewer evaluates this publication before release."}