{"type":"trials","record":{"id":9498,"source_id":"clinicaltrials-gov","external_id":"NCT07828847","title":"Involvement of Muscle Mitochondrial Dysfunction in Frailty in Older Adults. Role of Exercise","brief_summary":"This study aims to investigate the role of skeletal muscle mitochondrial dysfunction in the development of frailty in older adults and to evaluate whether a short-term, supervised resistance training intervention can improve skeletal muscle health and physical function. Frailty is a common age-related condition associated with increased vulnerability to disability, hospitalisation, and loss of independence. It is characterised by features that may include weakness, fatigue, reduced physical activity, and slower walking speed. Mitochondrial dysfunction in skeletal muscle may contribute to the development of frailty and may be further influenced by chronic conditions such as type 2 diabetes mellitus (T2DM), which are associated with metabolic alterations, inflammation, oxidative stress, and insulin resistance. Resistance training is an effective strategy to counteract age-related declines in muscle strength and physical function. However, the cellular and molecular mechanisms linking resistance training to improvements in mitochondrial function, skeletal muscle health, and resilience in older adults remain incompletely understood. The study will include approximately 120 participants comprising older adults with T2DM, healthy older adults, and young healthy controls. All participants will complete a 6-week supervised resistance training intervention, consisting of two sessions per week. Assessments performed before and after the intervention will include body composition, muscle morphology, physical performance, muscle strength, frailty, and blood-based biomarkers. Peripheral blood mononuclear cells (PBMCs) will be used to assess mitochondrial function, and plasma samples will be used for proteomic profiling. Stool samples will be collected for analysis of gut microbiota. A subset of participants will undergo skeletal muscle biopsies of the vastus lateralis at baseline and after the intervention. Muscle samples will be used to assess mitochondrial oxidative phosphorylation capacity, mitochondrial quality-control proteins, muscle fibre morphology, cell-type-specific transcriptomic profiles using single-nucleus RNA sequencing, and chromatin accessibility using ATAC-seq. The study will provide an integrated assessment of the effects of resistance training on mitochondrial function, skeletal muscle biology, physical function, frailty, circulating biomarkers, and gut microbiota. These findings may help clarify the biological mechanisms through which resistance training influences muscle health and resilience during ageing and in older adults with T2DM.","overall_status":"Recruiting","phases":[],"study_type":"INTERVENTIONAL","sponsor":"Fundación para la Investigación del Hospital Clínico de Valencia","enrollment":120,"countries":["Spain"],"start_date":"2022-01-29","completion_date":"2028-07-31","last_update_date":"2026-09-18","source_url":"https://clinicaltrials.gov/study/NCT07828847","editorial_summary":"A registered study indexed because it matched monitored longevity research terms. Registry status: Recruiting. Registration does not establish safety or effectiveness.","first_seen_at":"2026-09-18T12:17:29.245468+00:00","last_seen_at":"2026-09-25T06:15:54.38+00:00","metadata":{"sex":"ALL","acronym":"MITOSTRENGTH","age_range":"18 Years to 83 Years","comparator":"Group of healthy trained old people; Group of type 2 diabetic trained old patients; Group of healthy trained young people","organization":"Fundación para la Investigación del Hospital Clínico de Valencia","interventions":["Short-duration resistance training"],"registry_source":"ClinicalTrials.gov","outcome_measures":["Change in Mitochondrial Oxidative Phosphorylation (OXPHOS) Capacity in Skeletal Muscle — Baseline and after 6 weeks of…","Change in Mitochondrial Oxidative Phosphorylation (OXPHOS) Capacity in Peripheral Blood Mononuclear Cells (PBMCs) — Bas…","Change in Mitochondrial Quality Control Protein Expression in Skeletal Muscle — Baseline and after 6 weeks of supervise…","Change in Whole-Body Lean Mass — Baseline and after 6 weeks of supervised resistance training","Change in Whole-Body Fat Mass — Baseline and after 6 weeks of supervised resistance training","Change in 6-Minute Walk Test Distance — Baseline and after 6 weeks of supervised resistance training","Change in Blood Glucose-6-Phosphate Dehydrogenase (G6PD) Activity — Baseline and after 6 weeks of supervised resistance…","Change in Skeletal Muscle Cell-Type-Specific Transcriptomic Profiles — Baseline and after 6 weeks of supervised resista…","Change in Gut Microbiota Alpha Diversity — Baseline and after 6 weeks of supervised resistance training","Change in Quadriceps Muscle Thickness — Baseline and after 6 weeks of supervised resistance training","Change in Skeletal Muscle Fibre Cross-Sectional Area — Baseline and after 6 weeks of supervised resistance training","Change in Short Physical Performance Battery (SPPB) Score — Baseline and after 6 weeks of supervised resistance training","Change in FallSkip Test Performance Time — Baseline and after 6 weeks of supervised resistance training","Change in Blood Malondialdehyde (MDA) Levels — Baseline and after 6 weeks of supervised resistance training","Change in Fried Frailty Phenotype Score — Baseline and after 6 weeks of supervised resistance training","Change in Frailty Classification Assessed by the Survey of Health, Ageing and Retirement in Europe Frailty Instrument (…","Change in Quadriceps Extension 3-Repetition Maximum (3RM) — Baseline and after 6 weeks of supervised resistance training","Change in Skeletal Muscle Chromatin Accessibility Profiles Assessed by ATAC-seq — Baseline and after 6 weeks of supervi…","Change in Plasma Proteomic Profiles — Baseline and after 6 weeks of supervised resistance training"],"design_description":"NON RANDOMIZED · PARALLEL · TREATMENT · SINGLE","source_has_results":false},"controlled_terms":["Diabetes Type 2","Frail Elderly Syndrome","Mitochondrial Function, Bioenergetics","Functionality","Short-duration resistance training"],"relevance_confidence":100,"source_quality_score":100,"freshness_score":100,"publication_state":"published","match_explanation":"Title contains controlled term: mitochondrial dysfunction.","quality_checked_at":"2026-09-25T06:16:07.48358+00:00","duplicate_cluster_key":"id:nct07828847","duplicate_of_id":null,"evidence_snapshot":{"status":"structured","version":1,"duration":"2022-01-29 to 2028-07-31","comparator":"Group of healthy trained old people; Group of type 2 diabetic trained old patients; Group of healthy trained young people","confidence":"structured-source","population":"ALL · 18 Years to 83 Years","provenance":{"duration":"start_date and completion_date","comparator":"registry arm fields","population":"registry eligibility fields","intervention":"registry intervention fields","participants":"enrollment","study_design":"study_type and registry design fields","evidence_stage":"phases","reported_outcome":"not available","outcomes_measured":"registry outcome-measure fields"},"generated_at":"2026-09-25T06:15:54.383Z","intervention":["Short-duration resistance training"],"participants":120,"study_design":"NON RANDOMIZED · PARALLEL · TREATMENT · SINGLE","subject_scope":"Human clinical study registration","evidence_stage":"Phase not reported","safety_context":"Eligibility, adverse-event details, and clinical decisions must be checked in the official registry and with qualified clinicians.","source_support":"Structured registry protocol metadata; no finding-level conclusion is generated.","main_limitation":"This is a study registration. No reusable structured result is available here, so it cannot show whether the intervention worked or was safe.","reported_outcome":null,"outcomes_measured":["Change in Mitochondrial Oxidative Phosphorylation (OXPHOS) Capacity in Skeletal Muscle — Baseline and after 6 weeks of…","Change in Mitochondrial Oxidative Phosphorylation (OXPHOS) Capacity in Peripheral Blood Mononuclear Cells (PBMCs) — Bas…","Change in Mitochondrial Quality Control Protein Expression in Skeletal Muscle — Baseline and after 6 weeks of supervise…","Change in Whole-Body Lean Mass — Baseline and after 6 weeks of supervised resistance training","Change in Whole-Body Fat Mass — Baseline and after 6 weeks of supervised resistance training","Change in 6-Minute Walk Test Distance — Baseline and after 6 weeks of supervised resistance training","Change in Blood Glucose-6-Phosphate Dehydrogenase (G6PD) Activity — Baseline and after 6 weeks of supervised resistance…","Change in Skeletal Muscle Cell-Type-Specific Transcriptomic Profiles — Baseline and after 6 weeks of supervised resista…","Change in Gut Microbiota Alpha Diversity — Baseline and after 6 weeks of supervised resistance training","Change in Quadriceps Muscle Thickness — Baseline and after 6 weeks of supervised resistance training","Change in Skeletal Muscle Fibre Cross-Sectional Area — Baseline and after 6 weeks of supervised resistance training","Change in Short Physical Performance Battery (SPPB) Score — Baseline and after 6 weeks of supervised resistance training","Change in FallSkip Test Performance Time — Baseline and after 6 weeks of supervised resistance training","Change in Blood Malondialdehyde (MDA) Levels — Baseline and after 6 weeks of supervised resistance training","Change in Fried Frailty Phenotype Score — Baseline and after 6 weeks of supervised resistance training","Change in Frailty Classification Assessed by the Survey of Health, Ageing and Retirement in Europe Frailty Instrument (…","Change in Quadriceps Extension 3-Repetition Maximum (3RM) — Baseline and after 6 weeks of supervised resistance training","Change in Skeletal Muscle Chromatin Accessibility Profiles Assessed by ATAC-seq — Baseline and after 6 weeks of supervi…","Change in Plasma Proteomic Profiles — Baseline and after 6 weeks of supervised resistance training"],"regulatory_context":"Trial registration is not regulatory approval and does not establish that an intervention is available."},"clinical_trial_topics":[{"topic_slug":"exercise","is_published":true,"match_reasons":["Title contains controlled term: exercise.","Abstract contains controlled term: physical activity.","Title supplies longevity context: frailty, frail.","Study type is explicitly identified as INTERVENTIONAL."],"matched_fields":["title","abstract","title context","study type"],"relevance_score":100,"intelligence_topics":{"name":"Exercise","slug":"exercise"}},{"topic_slug":"frailty","is_published":true,"match_reasons":["Title contains controlled term: frailty.","Abstract contains controlled term: frailty.","Title supplies longevity context: frailty, frail.","Study type is explicitly identified as INTERVENTIONAL."],"matched_fields":["title","abstract","title context","study type"],"relevance_score":100,"intelligence_topics":{"name":"Frailty","slug":"frailty"}},{"topic_slug":"mitochondrial-function","is_published":true,"match_reasons":["Title contains controlled term: mitochondrial dysfunction.","Abstract contains controlled term: mitochondrial dysfunction, mitochondrial function.","Source terminology contains: mitochondrial function.","Title supplies longevity context: frailty, frail.","Study type is explicitly identified as INTERVENTIONAL."],"matched_fields":["title","abstract","controlled terminology","title context","study type"],"relevance_score":100,"intelligence_topics":{"name":"Mitochondrial function","slug":"mitochondrial-function"}}],"content_sources":{"name":"ClinicalTrials.gov","homepage_url":"https://clinicaltrials.gov/"}},"canonical_url":"https://www.immortal.life/trials/9498","automation_disclosure":"Generated automatically from cited source metadata. No scientist, clinician, researcher, editor, or human reviewer evaluates this publication before release."}